Quiz on Agrobacterium Tumefaciens

Agarobacterium tumefaciensis a soil borne, gram negative, rod shapes, motile bacteria which infect the wounded sites of the plant. It is the natural vector which can transfer DNA into the cell and due to this characteristic feature it is a very important method of gene transfer in plants.

So, this quiz is about this bacterium. A score of around 80% will ensure that you have good knowledge about this topic.

More than one option can be correct in the given questions.

Questions:

1. Which type of plant does it naturally infects?
   1. Monocotyledon
   2. Dicotyledonous
   3. Both a and b
   4. None of the above
2. Where it is commonly found?
   1. In the stem of plant
   2. Rhizosphere
   3. Within roots
   4. None of the above
3. What disease it cause in the infected plants?
   1. Crown gall
   2. Necrosis
   3. Hairy roots
   4. None of the above
4. What is released by wounded cells of plants?
   1. Sugar
   2. Phenolic compounds like acetosyringone
   3. Proteins
   4. Lipids
5. How it moves towards wounded sites?
   1. By rotation
   2. By turbid movement
   3. By chemotaxis
   4. None of the above
6. Which plasmid is present in this bacterium?
   1. Tumor inducing plasmid (Ti-plasmid)
   2. R-plasmid
   3. F-plasmid
   4. None of the above
7. Which part of plasmid is transferred into the plant cell?
   1. vir region
   2. T-DNA
   3. Replication site
   4. Metabolism genes
8. Which of the following is the feature of plasmid?
   1. Contain one or more T-DNA regions
   2. Contain vir region
   3. Contain origin of replication
   4. Contain a region enabling conjugative transfer
   5. Contain genes for opines catabolism
9. What is the size of plasmid?
   1. 100kb
   2. 150kb
   3. 200kb
   4. 250kb
10. What is the size of T-DNA region in octopine strains?
    1. 20kb
    2. 14kb
    3. 7kb
    4. All of the above
11. What is the size of T-DNA in nopaline strains?
    1. 20kb
    2. 14kb
    3. 7kb
    4. All of the above
12. What is the size of border sequences?
    1. 23 base pairs
    2. 24 base pairs
    3. 25 base pairs
    4. 26 base pairs
13. Which are the common genes which are coded by the T-DNA region?
    1. auxA and auxB for synthesis of enzyme tryptophan monooxygenase and indole acetamidehydrolase
    2. cyt for synthesis of isopropyl transferase
    3. tm1 region for regulating tumor size
    4. ocs for synthesis of octopine
14. Why crown gall is formed?
    1. It is because of the release of auxins and cytokinins by the transformed plant cells
    2. It is because of the release of opines and agropines
    3. Both a and b
    4. None of the above
15. Which region is responsible for transfer of T-DNA?
    1. Replication region
    2. vir region
    3. Both a and b
    4. None of the above
16. What is the size of vir region?
    1. 30 kb
    2. 20kb
    3. 40 kb
    4. 60kb
17. How many genes does vir region encode?
    1. At least 8
    2. 8
    3. 9
    4. At least 9
18. Which genes are responsible for attachment of bacterium to plant cell?
19. chvA
20. chvB
21. pscA
22. attR
23. How many steps does the attachment procedure include?
    1. 3
    2. 4
    3. 1
    4. 2
24. Name the chromosomal genes involved in attachment?
25. chvA
26. chvB
27. pscA
28. attR
29. Name the chromosomal gene which helps in vir-region induction?
30. chvA
31. chvB
32. chvF
33. chvE
34. How T-DNA gets transported across the bacterial cell wall?
    1. They get transported through T-pilus
    2. Bacterial secretory system
    3. By vesicles
    4. By virB operon and VirD4 proteins
35. Which plant proteins are responsible to facilitate the entry of T-DNA through nuclear pore complex (NPC)?
    1. Export proteins
    2. Nuclease
    3. Esterase
    4. Importins
36. What type of recombination does it takes place?
    1. Homologous recombination
    2. Protein mediated recombination
    3. Illegitimate recombination
    4. None of the above
37. How many nuclear localization signals (NLS) are present on protein VirE2?
    1. 1
    2. 2
    3. 3
    4. 4

Answers:

1. b
2. b
3. a
4. a, b
5. c
6. a
7. b
8. a, ,b, c, d, e
9. c
10. a
11. b, c
12. b
13. a, b, c, d
14. a
15. b
16. c
17. d
18. a, b, c, d
19. d
20. a, b, c
21. d
22. a, b, d
23. d
24. c
25. b

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